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Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants.

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  • Additional Information
    • Abstract:
      Expressed sequence tags (EST) are potential source for the development of genic microsatellite markers, gene discovery, comparative genomics, and other genomic studies. In the present study, 7630 ESTs were examined from NCBI for SSR identification and characterization. A total of 263 SSRs were identified with an average density of one SSR/4.2 kb (3.4% frequency). Analysis revealed that trinucleotide repeats (47.52%) were most abundant followed by tetranucleotide (19.77%), dinucleotide (19.01%), pentanucleotide (9.12%), and hexanucleotide repeats (4.56%). Functional annotation was done through homology search and gene ontology, and 35 EST-SSRs were selected. Primer pairs were designed for evaluation of cross transferability and polymorphism among 11 plants belonging to five different families. Total 402 alleles were generated at 155 loci with an average of 2.6 alleles/locus and the polymorphic information content (PIC) ranged from 0.15 to 0.92 with an average of 0.75. The cross transferability ranged from 34.84% to 98.06% in different plants, with an average of 67.86%. Thus, the validation study of annotated 35 EST-SSR markers which correspond to particular metabolic activity revealed polymorphism and evolutionary nature in different families of Angiospermic plants. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of International Journal of Genomics is the property of Hindawi Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Botany, University of Rajasthan, Jaipur 302004, India
      2Department of Biosciences, Manipal University, Jaipur 302007, India
      3Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur 302006, India
    • ISSN:
      2314-436X
    • Accession Number:
      10.1155/2014/863948
    • Accession Number:
      102124787
  • Citations
    • ABNT:
      UL HAQ, S. et al. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics, [s. l.], p. 1–12, 2014. DOI 10.1155/2014/863948. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=102124787&custid=s8280428. Acesso em: 13 jul. 2020.
    • AMA:
      ul Haq S, Jain R, Sharma M, Kachhwaha S, Kothari SL. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics. January 2014:1-12. doi:10.1155/2014/863948.
    • AMA11:
      ul Haq S, Jain R, Sharma M, Kachhwaha S, Kothari SL. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics. Published online January 2014:1-12. doi:10.1155/2014/863948
    • APA:
      ul Haq, S., Jain, R., Sharma, M., Kachhwaha, S., & Kothari, S. L. (2014). Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics, 1–12. https://doi.org/10.1155/2014/863948
    • Chicago/Turabian: Author-Date:
      ul Haq, Shamshad, Rohit Jain, Meenakshi Sharma, Sumita Kachhwaha, and S. L. Kothari. 2014. “Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants.” International Journal of Genomics, January, 1–12. doi:10.1155/2014/863948.
    • Harvard:
      ul Haq, S. et al. (2014) ‘Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants’, International Journal of Genomics, pp. 1–12. doi: 10.1155/2014/863948.
    • Harvard: Australian:
      ul Haq, S, Jain, R, Sharma, M, Kachhwaha, S & Kothari, SL 2014, ‘Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants’, International Journal of Genomics, pp. 1–12, viewed 13 July 2020, .
    • MLA:
      ul Haq, Shamshad, et al. “Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants.” International Journal of Genomics, Jan. 2014, pp. 1–12. EBSCOhost, doi:10.1155/2014/863948.
    • Chicago/Turabian: Humanities:
      ul Haq, Shamshad, Rohit Jain, Meenakshi Sharma, Sumita Kachhwaha, and S. L. Kothari. “Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants.” International Journal of Genomics, January 2014, 1–12. doi:10.1155/2014/863948.
    • Vancouver/ICMJE:
      ul Haq S, Jain R, Sharma M, Kachhwaha S, Kothari SL. Identification and Characterization of Microsatellites in Expressed Sequence Tags and Their Cross Transferability in Different Plants. International Journal of Genomics [Internet]. 2014 Jan [cited 2020 Jul 13];1–12. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=102124787&custid=s8280428