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RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress.

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  • Additional Information
    • Author-Supplied Keywords:
      Citrullus colocynthis
      Genetic diversity
      Heterozygosity (H)
      ISSR
      Marker index (MI)
      Multiplex ratio (MR)
      Polymorphic information content (PIC)
      Polymorphism
      RAPD
      Resolution power (RP)
      Similarity matrix
    • Abstract:
      Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) shows high levels of variation in fruit color, fruit stripe pattern, seed coat color, and size. Thirty-eight accessions of C. colocynthis plants from different parts of semi-arid Rajasthan were collected and genetic diversity was assessed using random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Out of 65 RAPD decamer primers, 50 primers produced 549 scorable bands of which 318 were polymorphic. Polymorphic banding patterns with the number of amplified fragments varied from 5 (OPA-08 and OPF-9) to 19 (OPT-20) in the molecular size range of 150-6000 bp. Percent polymorphism ranged from 22.2% (OPA-09) to 83.3% (OPE-12) with 55.14% polymorphism. Out of the 20 ISSR primers screened, 13 primers produced 166 amplification products, of which 99 were polymorphic. The number of bands amplified per primer varied between 9 (UBC-807, 802) and 16 (UBC-803, 812) with average band size between 250 and 4000 bp. Percent polymorphism ranged from 45.4% (UBC-815) to 73.3% (UBC-814) with 65.05% polymorphism. Dendrogram constructed on the basis of RAPD + ISSR polymorphism separated the accessions into four distinct clusters at 72% variation with Jaccard's similarity coefficient ranging from minimum 0.64 to 0.95. The matrices for RAPD and ISSR were also compared using Mantel's test and obtained correlation value ( r = 0.7947). Discriminating power of RAPD and ISSR markers was assessed by calculating polymorphic information content, multiplex ratio, marker index, and resolving power. Approx. 50% RAPD and ISSR markers showed PIC value and heterozygosity ( H) ≥ 0.50, indicating marker as informative. The primers that showed higher polymorphism had higher RP, MR, and MI values. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of 3 Biotech is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Department of Botany , University of Rajasthan , Jaipur India
    • ISSN:
      2190-572X
    • Accession Number:
      10.1007/s13205-017-0918-z
    • Accession Number:
      124830479
  • Citations
    • ABNT:
      VERMA, K. et al. RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech, [s. l.], v. 7, n. 5, p. 1–24, 2017. DOI 10.1007/s13205-017-0918-z. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=124830479&custid=s8280428. Acesso em: 24 fev. 2020.
    • AMA:
      Verma K, ul Haq S, Kachhwaha S, Kothari S. RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech. 2017;7(5):1-24. doi:10.1007/s13205-017-0918-z.
    • APA:
      Verma, K., ul Haq, S., Kachhwaha, S., & Kothari, S. (2017). RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech, 7(5), 1–24. https://doi.org/10.1007/s13205-017-0918-z
    • Chicago/Turabian: Author-Date:
      Verma, Kumar, Shamshad ul Haq, Sumita Kachhwaha, and S. Kothari. 2017. “RAPD and ISSR Marker Assessment of Genetic Diversity in Citrullus Colocynthis (L.) Schrad: A Unique Source of Germplasm Highly Adapted to Drought and High-Temperature Stress.” 3 Biotech 7 (5): 1–24. doi:10.1007/s13205-017-0918-z.
    • Harvard:
      Verma, K. et al. (2017) ‘RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress’, 3 Biotech, 7(5), pp. 1–24. doi: 10.1007/s13205-017-0918-z.
    • Harvard: Australian:
      Verma, K, ul Haq, S, Kachhwaha, S & Kothari, S 2017, ‘RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress’, 3 Biotech, vol. 7, no. 5, pp. 1–24, viewed 24 February 2020, .
    • MLA:
      Verma, Kumar, et al. “RAPD and ISSR Marker Assessment of Genetic Diversity in Citrullus Colocynthis (L.) Schrad: A Unique Source of Germplasm Highly Adapted to Drought and High-Temperature Stress.” 3 Biotech, vol. 7, no. 5, Aug. 2017, pp. 1–24. EBSCOhost, doi:10.1007/s13205-017-0918-z.
    • Chicago/Turabian: Humanities:
      Verma, Kumar, Shamshad ul Haq, Sumita Kachhwaha, and S. Kothari. “RAPD and ISSR Marker Assessment of Genetic Diversity in Citrullus Colocynthis (L.) Schrad: A Unique Source of Germplasm Highly Adapted to Drought and High-Temperature Stress.” 3 Biotech 7, no. 5 (August 24, 2017): 1–24. doi:10.1007/s13205-017-0918-z.
    • Vancouver/ICMJE:
      Verma K, ul Haq S, Kachhwaha S, Kothari S. RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress. 3 Biotech [Internet]. 2017 Aug 24 [cited 2020 Feb 24];7(5):1–24. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=124830479&custid=s8280428