Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants.

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • Author-Supplied Keywords:
      Artificial gene amplification and extension
      Bacteria
      Biology and life sciences
      Biomolecular isolation
      DNA isolation
      Ecology
      Ecology and environmental sciences
      Eukaryota
      Flowering plants
      Fungal pathogens
      Grapevine
      Medical microbiology
      Medicine and health sciences
      Microbial pathogens
      Microbiology
      Molecular biology
      Molecular biology techniques
      Mycology
      Organisms
      Pathogens
      Pathology and laboratory medicine
      Plant anatomy
      Plant ecology
      Plant science
      Plant-environment interactions
      Plants
      Polymerase chain reaction
      Random amplified polymorphic DNA technique
      Research and analysis methods
      Research Article
      Rhizosphere
      Streptomyces
      Wood
    • NAICS/Industry Codes:
      111332 Grape Vineyards
      413150 Fresh fruit and vegetable merchant wholesalers
    • Abstract:
      The endophytic Streptomyces sp. VV/E1, and rhizosphere Streptomyces sp. VV/R4 strains, isolated from grapevine plants were shown in a previous work to reduce the infection rate of fungal pathogens involved in young grapevine decline. In this study we cloned fragments from randomly amplified polymorphic DNA (RAPD), and developed two stably diagnostic sequence-characterized amplified region (SCAR) markers of 182 and 160 bp for the VV/E1 and VV/R4 strains, respectively. The SCAR markers were not found in another 50 actinobacterial strains isolated from grapevine plants. Quantitative real-time PCR protocols based on the amplification of these SCAR markers were used for the detection and quantification of both strains in plant material. These strains were applied on young potted plants using two methods: perforation of the rootstock followed by injection of the microorganisms or soaking the root system in a bacterial suspension. Both methods were combined with a booster treatment by direct addition of a bacterial suspension to the soil near the root system. Analysis of uprooted plants showed that those inoculated by injection exhibited the highest rate of colonization. In contrast, direct addition of either strain to the soil did not lead to reliable colonization. This study has developed molecular tools for analyzing different methods for inoculating grapevine plants with selected Streptomyces sp. strains which protect them from fungal infections that enter through their root system. These tools are of great applied interest since they could easily be established in nurseries to produce grafted grapevine plants that are protected against fungal pathogens. Finally, this methodology might also be applied to other vascular plants for their colonization with beneficial biological control agents. [ABSTRACT FROM AUTHOR]
    • Abstract:
      Copyright of PLoS ONE is the property of Public Library of Science and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
    • Author Affiliations:
      1Instituto de Investigación de la Viña y el Vino, Universidad de León, León, Spain
      2Área de Genética, Departamento de Biología Molecular, Universidad de León, León, Spain
      3RGA Bio-Investigación S.L., León, Spain
    • Full Text Word Count:
      8565
    • ISSN:
      1932-6203
    • Accession Number:
      10.1371/journal.pone.0211225
    • Accession Number:
      134268056
  • Citations
    • ABNT:
      GONZÁLEZ-GARCÍA, S. et al. Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants. PLoS ONE, [s. l.], v. 14, n. 1, p. 1–18, 2019. Disponível em: . Acesso em: 14 out. 2019.
    • AMA:
      González-García S, Álvarez-Pérez JM, Sáenz de Miera LE, et al. Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants. PLoS ONE. 2019;14(1):1-18. doi:10.1371/journal.pone.0211225.
    • APA:
      González-García, S., Álvarez-Pérez, J. M., Sáenz de Miera, L. E., Cobos, R., Ibañez, A., Díez-Galán, A., … Coque, J. J. R. (2019). Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants. PLoS ONE, 14(1), 1–18. https://doi.org/10.1371/journal.pone.0211225
    • Chicago/Turabian: Author-Date:
      González-García, Sandra, Jose Manuel Álvarez-Pérez, Luis E. Sáenz de Miera, Rebeca Cobos, Ana Ibañez, Alba Díez-Galán, Enrique Garzón-Jimeno, and Juan José R. Coque. 2019. “Developing Tools for Evaluating Inoculation Methods of Biocontrol Streptomyces Sp. Strains into Grapevine Plants.” PLoS ONE 14 (1): 1–18. doi:10.1371/journal.pone.0211225.
    • Harvard:
      González-García, S. et al. (2019) ‘Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants’, PLoS ONE, 14(1), pp. 1–18. doi: 10.1371/journal.pone.0211225.
    • Harvard: Australian:
      González-García, S, Álvarez-Pérez, JM, Sáenz de Miera, LE, Cobos, R, Ibañez, A, Díez-Galán, A, Garzón-Jimeno, E & Coque, JJR 2019, ‘Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants’, PLoS ONE, vol. 14, no. 1, pp. 1–18, viewed 14 October 2019, .
    • MLA:
      González-García, Sandra, et al. “Developing Tools for Evaluating Inoculation Methods of Biocontrol Streptomyces Sp. Strains into Grapevine Plants.” PLoS ONE, vol. 14, no. 1, Jan. 2019, pp. 1–18. EBSCOhost, doi:10.1371/journal.pone.0211225.
    • Chicago/Turabian: Humanities:
      González-García, Sandra, Jose Manuel Álvarez-Pérez, Luis E. Sáenz de Miera, Rebeca Cobos, Ana Ibañez, Alba Díez-Galán, Enrique Garzón-Jimeno, and Juan José R. Coque. “Developing Tools for Evaluating Inoculation Methods of Biocontrol Streptomyces Sp. Strains into Grapevine Plants.” PLoS ONE 14, no. 1 (January 24, 2019): 1–18. doi:10.1371/journal.pone.0211225.
    • Vancouver/ICMJE:
      González-García S, Álvarez-Pérez JM, Sáenz de Miera LE, Cobos R, Ibañez A, Díez-Galán A, et al. Developing tools for evaluating inoculation methods of biocontrol Streptomyces sp. strains into grapevine plants. PLoS ONE [Internet]. 2019 Jan 24 [cited 2019 Oct 14];14(1):1–18. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=asn&AN=134268056&custid=s8280428