A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor.

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • Publication Information:
      Public Library of Science (PLoS).
    • Collection:
      LCC:Medicine
      LCC:Science
    • Abstract:
      Ochratoxin A(OTA) is found to be one of the predominant contaminating mycotoxins in a wide variety of food commodities. To avoid the risk of OTA consumption, the detection and quantitation of OTA level are of great significance. Based on the fact that ssDNA aptamer has the ability to form a double-strand structure with its complementary sequence, a simple and rapid aptamer-based label-free approach for highly sensitive and selective fluorescence detection of OTA was developed by using ultra-sensitive double-strand DNA specific dyes PicoGreen. The results showed that as low as 1 ng/mL of OTA could be detected with a dynamic range of more than 5 orders of magnitude which satisfies the requirements for OTA maximum residue limit in various food regulated by European Commission. With the specificity of aptamer, the assay exhibited high selectivity for OTA against two other analogues (N-acetyl-l-phenylalanine and zearalenone). We also tested the aptasensor practicability using real sample of 1% beer spiked with a series of concentration of OTA and the results show good tolerance to matrix effect. All detections could be achieved in less than 30 min, which provides a simple, quick and sensitive detection method for OTA screening in food safety and could be easily extend to other small molecular chemical compounds detection which aptamer has been selected.
    • File Description:
      electronic resource
    • ISSN:
      1932-6203
    • Relation:
      http://europepmc.org/articles/PMC3897567?pdf=render; https://doaj.org/toc/1932-6203
    • Accession Number:
      10.1371/journal.pone.0085968
    • Rights:
      Journal Licence: CC BY
    • Accession Number:
      edsdoj.209c6167680447eb8be4078536577834
  • Citations
    • ABNT:
      ZHENZHEN LV et al. A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE, [s. l.], n. 1, p. e85968, [s.d.]. Disponível em: . Acesso em: 17 set. 2019.
    • AMA:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, et al. A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE. (1):e85968. doi:10.1371/journal.pone.0085968.
    • APA:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, … Cheng Li. (n.d.). A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE, (1), e85968. https://doi.org/10.1371/journal.pone.0085968
    • Chicago/Turabian: Author-Date:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang, and Cheng Li. 2019. “A Simple and Sensitive Approach for Ochratoxin A Detection Using a Label-Free Fluorescent Aptasensor.” PLoS ONE, no. 1: e85968. Accessed September 17. doi:10.1371/journal.pone.0085968.
    • Harvard:
      Zhenzhen Lv et al. (no date) ‘A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor’, PLoS ONE, (1), p. e85968. doi: 10.1371/journal.pone.0085968.
    • Harvard: Australian:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang & Cheng Li n.d., ‘A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor’, PLoS ONE, no. 1, p. e85968, viewed 17 September 2019, .
    • MLA:
      Zhenzhen Lv, et al. “A Simple and Sensitive Approach for Ochratoxin A Detection Using a Label-Free Fluorescent Aptasensor.” PLoS ONE, no. 1, p. e85968. EBSCOhost, doi:10.1371/journal.pone.0085968. Accessed 17 Sept. 2019.
    • Chicago/Turabian: Humanities:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang, and Cheng Li. “A Simple and Sensitive Approach for Ochratoxin A Detection Using a Label-Free Fluorescent Aptasensor.” PLoS ONE, no. 1: e85968. Accessed September 17, 2019. doi:10.1371/journal.pone.0085968.
    • Vancouver/ICMJE:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, et al. A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE [Internet]. [cited 2019 Sep 17];(1):e85968. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.209c6167680447eb8be4078536577834&custid=s8280428