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A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor.

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  • Additional Information
    • Publication Information:
      Public Library of Science (PLoS), 2014.
    • Publication Date:
      2014
    • Collection:
      LCC:Medicine
      LCC:Science
    • Abstract:
      Ochratoxin A(OTA) is found to be one of the predominant contaminating mycotoxins in a wide variety of food commodities. To avoid the risk of OTA consumption, the detection and quantitation of OTA level are of great significance. Based on the fact that ssDNA aptamer has the ability to form a double-strand structure with its complementary sequence, a simple and rapid aptamer-based label-free approach for highly sensitive and selective fluorescence detection of OTA was developed by using ultra-sensitive double-strand DNA specific dyes PicoGreen. The results showed that as low as 1 ng/mL of OTA could be detected with a dynamic range of more than 5 orders of magnitude which satisfies the requirements for OTA maximum residue limit in various food regulated by European Commission. With the specificity of aptamer, the assay exhibited high selectivity for OTA against two other analogues (N-acetyl-l-phenylalanine and zearalenone). We also tested the aptasensor practicability using real sample of 1% beer spiked with a series of concentration of OTA and the results show good tolerance to matrix effect. All detections could be achieved in less than 30 min, which provides a simple, quick and sensitive detection method for OTA screening in food safety and could be easily extend to other small molecular chemical compounds detection which aptamer has been selected.
    • File Description:
      electronic resource
    • ISSN:
      1932-6203
    • Relation:
      http://europepmc.org/articles/PMC3897567?pdf=render; https://doaj.org/toc/1932-6203
    • Accession Number:
      10.1371/journal.pone.0085968
    • Rights:
      Journal Licence: CC BY
    • Accession Number:
      edsdoj.209c6167680447eb8be4078536577834
  • Citations
    • ABNT:
      ZHENZHEN LV et al. A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE, [s. l.], v. 9, n. 1, p. e85968, 2014. DOI 10.1371/journal.pone.0085968. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.209c6167680447eb8be4078536577834&custid=s8280428. Acesso em: 15 ago. 2020.
    • AMA:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, et al. A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE. 2014;9(1):e85968. doi:10.1371/journal.pone.0085968
    • APA:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang, & Cheng Li. (2014). A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE, 9(1), e85968. https://doi.org/10.1371/journal.pone.0085968
    • Chicago/Turabian: Author-Date:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang, and Cheng Li. 2014. “A Simple and Sensitive Approach for Ochratoxin A Detection Using a Label-Free Fluorescent Aptasensor.” PLoS ONE 9 (1): e85968. doi:10.1371/journal.pone.0085968.
    • Harvard:
      Zhenzhen Lv et al. (2014) ‘A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor’, PLoS ONE, 9(1), p. e85968. doi: 10.1371/journal.pone.0085968.
    • Harvard: Australian:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang & Cheng Li 2014, ‘A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor’, PLoS ONE, vol. 9, no. 1, p. e85968, viewed 15 August 2020, .
    • MLA:
      Zhenzhen Lv, et al. “A Simple and Sensitive Approach for Ochratoxin A Detection Using a Label-Free Fluorescent Aptasensor.” PLoS ONE, vol. 9, no. 1, Jan. 2014, p. e85968. EBSCOhost, doi:10.1371/journal.pone.0085968.
    • Chicago/Turabian: Humanities:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, Shuming Yang, and Cheng Li. “A Simple and Sensitive Approach for Ochratoxin A Detection Using a Label-Free Fluorescent Aptasensor.” PLoS ONE 9, no. 1 (January 1, 2014): e85968. doi:10.1371/journal.pone.0085968.
    • Vancouver/ICMJE:
      Zhenzhen Lv, Ailiang Chen, Jinchuan Liu, Zheng Guan, Yu Zhou, Siyuan Xu, et al. A simple and sensitive approach for ochratoxin A detection using a label-free fluorescent aptasensor. PLoS ONE [Internet]. 2014 Jan 1 [cited 2020 Aug 15];9(1):e85968. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsdoj&AN=edsdoj.209c6167680447eb8be4078536577834&custid=s8280428