A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • Affiliation:
      a College of Biological Engineering, Jilin College of Agricultural Science and Technology, Jilin, Jilin 132101, China
      b Department of Neurology, The Third Hospital of Jilin University, Changchun, Jilin 130021, China
      c College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China
    • Keywords:
      PCR
      Sheep
      Strip
      Meat adulteration
    • Abstract:
      Molecular methods, such as PCR and real-time PCR, have been developed to detect species in meat and meat products. Despite good specificity and sensitivity, they are not widely implemented in food control programs due to complex operation or financial reasons. In the present study, a simple, rapid and affordable method, Sheep-PCR-Strip [Sheep specific polymerase chain reaction-Strip], was developed for the authentic identification of raw and heat-treated mutton. The assay is based on PCR amplification of sheep DNA, followed by detection of the PCR product by a strip format; the result can be read within 5 min by the naked eye. There is a real advantage of the strip approach rather in the reduced time (5 min versus electrophoresis) and avoidance of chemicals (e.g. ethidiumbromide). The sensitivity of the Sheep-PCR-Strip test was established to be 0.01% for the detection of adulterated meat; the limit of detection (LOD) was up to 0.01 pg of sheep DNA. The assay was also specific for sheep, and no cross-reactions were observed in other non-target species. It is a promising new tool for sheep identification and can be rapidly modified for other meat detection and widely used for solving problems related to food quality assurance, species authentication and traceability.
    • ISSN:
      0956-7135
    • Accession Number:
      10.1016/j.foodcont.2015.07.030
    • Accession Number:
      S0956713515301183
    • Copyright:
      Copyright © 2015 Elsevier Ltd All rights reserved.
  • Citations
    • ABNT:
      YIN, R. et al. A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat. Food Control, [s. l.], v. 60, p. 146–150, 2016. DOI 10.1016/j.foodcont.2015.07.030. Disponível em: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S0956713515301183&custid=s8280428. Acesso em: 9 dez. 2019.
    • AMA:
      Yin R, Sun Y, Yu S, et al. A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat. Food Control. 2016;60:146-150. doi:10.1016/j.foodcont.2015.07.030.
    • APA:
      Yin, R., Sun, Y., Yu, S., Wang, Y., Zhang, M., Xu, Y., … Xu, N. (2016). A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat. Food Control, 60, 146–150. https://doi.org/10.1016/j.foodcont.2015.07.030
    • Chicago/Turabian: Author-Date:
      Yin, Rui, Yajuan Sun, Shuang Yu, Yi Wang, Meiping Zhang, Yawei Xu, Jian Xue, and Na Xu. 2016. “A Validated Strip-Based Lateral Flow Assay for the Confirmation of Sheep-Specific PCR Products for the Authentication of Meat.” Food Control 60 (February): 146–50. doi:10.1016/j.foodcont.2015.07.030.
    • Harvard:
      Yin, R. et al. (2016) ‘A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat’, Food Control, 60, pp. 146–150. doi: 10.1016/j.foodcont.2015.07.030.
    • Harvard: Australian:
      Yin, R, Sun, Y, Yu, S, Wang, Y, Zhang, M, Xu, Y, Xue, J & Xu, N 2016, ‘A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat’, Food Control, vol. 60, pp. 146–150, viewed 9 December 2019, .
    • MLA:
      Yin, Rui, et al. “A Validated Strip-Based Lateral Flow Assay for the Confirmation of Sheep-Specific PCR Products for the Authentication of Meat.” Food Control, vol. 60, Feb. 2016, pp. 146–150. EBSCOhost, doi:10.1016/j.foodcont.2015.07.030.
    • Chicago/Turabian: Humanities:
      Yin, Rui, Yajuan Sun, Shuang Yu, Yi Wang, Meiping Zhang, Yawei Xu, Jian Xue, and Na Xu. “A Validated Strip-Based Lateral Flow Assay for the Confirmation of Sheep-Specific PCR Products for the Authentication of Meat.” Food Control 60 (February 1, 2016): 146–50. doi:10.1016/j.foodcont.2015.07.030.
    • Vancouver/ICMJE:
      Yin R, Sun Y, Yu S, Wang Y, Zhang M, Xu Y, et al. A validated strip-based lateral flow assay for the confirmation of sheep-specific PCR products for the authentication of meat. Food Control [Internet]. 2016 Feb 1 [cited 2019 Dec 9];60:146–50. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edselp&AN=S0956713515301183&custid=s8280428