A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension

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  • Additional Information
    • Publication Information:
      National Academy of Sciences, 2002.
    • Publication Date:
      2002
    • Abstract:
      We have examined the effect of neutrophil concentration on killing of a clinical isolate of Staphylococcus epidermidis. Human neutrophils at concentrations varying from 105to 107per ml were mixed in suspension with S. epidermidis at concentrations varying from 103to 108colony-forming units/ml, and the concentration of viable bacteria was assayed after various times at 37°C. The rate of bacterial killing depended on the concentration of neutrophils and not on the ratio of neutrophils to bacteria. Below a critical concentration of neutrophils, bacteria growth was greater than neutrophil killing of bacteria even when the ratio of neutrophils to bacteria was 100:1. We fitted the time course of bacterial concentration and its dependence on neutrophil concentration with an exponential function, the exponent of which is (-kp + g)t, where k is the second-order rate constant for bacterial killing, p is the neutrophil concentration, g is the first-order rate constant for bacterial growth, and t is time. We found that k ≈ 2 × 10-8ml per neutrophil per min, and g ≈ 8 × 10-3/min. Only when p is greater than g/k, which we call the critical neutrophil concentration, does the bacterial concentration fall. Under optimal assay conditions, the critical neutrophil concentration was 3-4 × 105per ml, a value very close to that (≤5 × 105per ml) known to predispose humans to bacterial and fungal infections.
    • ISSN:
      00278424
    • Rights:
      Copyright 1993-2002 National Academy of Sciences of the United States of America
    • Accession Number:
      edsjsr.3059001
  • Citations
    • ABNT:
      YONGMEI LI et al. A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension. Proceedings of the National Academy of Sciences of the United States of America, [s. l.], v. 99, n. 12, p. 8289, 2002. Disponível em: . Acesso em: 22 set. 2019.
    • AMA:
      Yongmei Li, Arthur Karlin, John D. Loike, Samuel C. Silverstein. A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension. Proceedings of the National Academy of Sciences of the United States of America. 2002;99(12):8289. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428. Accessed September 22, 2019.
    • APA:
      Yongmei Li, Arthur Karlin, John D. Loike, & Samuel C. Silverstein. (2002). A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension. Proceedings of the National Academy of Sciences of the United States of America, 99(12), 8289. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428
    • Chicago/Turabian: Author-Date:
      Yongmei Li, Arthur Karlin, John D. Loike, and Samuel C. Silverstein. 2002. “A Critical Concentration of Neutrophils Is Required for Effective Bacterial Killing in Suspension.” Proceedings of the National Academy of Sciences of the United States of America 99 (12): 8289. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428.
    • Harvard:
      Yongmei Li et al. (2002) ‘A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension’, Proceedings of the National Academy of Sciences of the United States of America, 99(12), p. 8289. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428 (Accessed: 22 September 2019).
    • Harvard: Australian:
      Yongmei Li, Arthur Karlin, John D. Loike & Samuel C. Silverstein 2002, ‘A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension’, Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 12, p. 8289, viewed 22 September 2019, .
    • MLA:
      Yongmei Li, et al. “A Critical Concentration of Neutrophils Is Required for Effective Bacterial Killing in Suspension.” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 12, 2002, p. 8289. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428.
    • Chicago/Turabian: Humanities:
      Yongmei Li, Arthur Karlin, John D. Loike, and Samuel C. Silverstein. “A Critical Concentration of Neutrophils Is Required for Effective Bacterial Killing in Suspension.” Proceedings of the National Academy of Sciences of the United States of America 99, no. 12 (2002): 8289. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428.
    • Vancouver/ICMJE:
      Yongmei Li, Arthur Karlin, John D. Loike, Samuel C. Silverstein. A Critical Concentration of Neutrophils is Required for Effective Bacterial Killing in Suspension. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2002 [cited 2019 Sep 22];99(12):8289. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3059001&custid=s8280428