Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device

Item request has been placed! ×
Item request cannot be made. ×
  Processing Request
  • Additional Information
    • Publication Information:
      National Academy of Sciences, 2004.
    • Publication Date:
      2004
    • Abstract:
      We demonstrate a technique for detecting magnetically labeled Listeria monocytogenes and for measuring the binding rate between antibody-linked magnetic particles and bacteria. This sensitive assay quantifies specific bacteria in a sample without the need to immobilize them or wash away unbound magnetic particles. In the measurement, we add 50-nm-diameter superparamagnetic magnetite particles, coated with antibodies, to an aqueous sample containing L. monocytogenes. We apply a pulsed magnetic field to align the magnetic dipole moments and use a high-transition temperature superconducting quantum interference device, an extremely sensitive detector of magnetic flux, to measure the magnetic relaxation signal when the field is turned off. Unbound particles randomize direction by Brownian rotation too quickly to be detected. In contrast, particles bound to L. monocytogenes are effectively immobilized and relax in about 1 s by rotation of the internal dipole moment. This Néel relaxation process is detected by the superconducting quantum interference device. The measurements indicate a detection limit of (5.6 ± 1.1) x 106L. monocytogenes in our sample volume of 20 μ l. If the sample volume were reduced to 1 nl, we estimate that the detection limit could be improved to 230 ± 40 L. monocytogenes cells. Time-resolved measurements yield the binding rate between the particles and bacteria.
    • ISSN:
      00278424
    • Rights:
      Copyright 1993-2004 National Academy of Sciences of the United States of America
    • Accession Number:
      edsjsr.3148385
  • Citations
    • ABNT:
      H. L. GROSSMAN et al. Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device. Proceedings of the National Academy of Sciences of the United States of America, [s. l.], v. 101, n. 1, p. 129, 2004. Disponível em: . Acesso em: 18 out. 2019.
    • AMA:
      H. L. Grossman, W. R. Myers, V. J. Vreeland, et al. Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(1):129. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428. Accessed October 18, 2019.
    • APA:
      H. L. Grossman, W. R. Myers, V. J. Vreeland, R. Bruehl, M. D. Alper, C. R. Bertozzi, & John Clarke. (2004). Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device. Proceedings of the National Academy of Sciences of the United States of America, 101(1), 129. Retrieved from http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428
    • Chicago/Turabian: Author-Date:
      H. L. Grossman, W. R. Myers, V. J. Vreeland, R. Bruehl, M. D. Alper, C. R. Bertozzi, and John Clarke. 2004. “Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device.” Proceedings of the National Academy of Sciences of the United States of America 101 (1): 129. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428.
    • Harvard:
      H. L. Grossman et al. (2004) ‘Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device’, Proceedings of the National Academy of Sciences of the United States of America, 101(1), p. 129. Available at: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428 (Accessed: 18 October 2019).
    • Harvard: Australian:
      H. L. Grossman, W. R. Myers, V. J. Vreeland, R. Bruehl, M. D. Alper, C. R. Bertozzi & John Clarke 2004, ‘Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device’, Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 1, p. 129, viewed 18 October 2019, .
    • MLA:
      H. L. Grossman, et al. “Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device.” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 1, 2004, p. 129. EBSCOhost, search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428.
    • Chicago/Turabian: Humanities:
      H. L. Grossman, W. R. Myers, V. J. Vreeland, R. Bruehl, M. D. Alper, C. R. Bertozzi, and John Clarke. “Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device.” Proceedings of the National Academy of Sciences of the United States of America 101, no. 1 (2004): 129. http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428.
    • Vancouver/ICMJE:
      H. L. Grossman, W. R. Myers, V. J. Vreeland, R. Bruehl, M. D. Alper, C. R. Bertozzi, et al. Detection of Bacteria in Suspension by Using a Superconducting Quantum Interference Device. Proceedings of the National Academy of Sciences of the United States of America [Internet]. 2004 [cited 2019 Oct 18];101(1):129. Available from: http://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsjsr&AN=edsjsr.3148385&custid=s8280428